Родина Скопових

Родина Сипухових

Вперше отримана ДНК з яєчної шкаралупи вимерлих птахів PDF Print Email
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Інтернаціональній команді вчених вдалося витягнути генетичний код зі скам'янілих яєць стародавніх пернатих. Керував роботою Майкл Бансе (Michael Bunce), голова генетичної лабораторії при австралійському університеті Мердока.

Фахівці працювали з яйцями декількох викопних птахів, серед яких були представники роду епіорнісових (Aepyornithidae), відомих як слонові птахи, - це одні з найбільших представників цього класу живих істот. 

Як правило, вилучення ДНК із скам'янілих останків ускладнюється надмірною кількістю сторонніх домішок. Через це колишні спроби отримати ДНК з кісток триметрової Aepyornis безпосередньо провалювалися. 

Однак, як повідомляється в прес-релізі, шкаралупа скам'янілих яєць (кожне в 160 разів більше курячого за обсягом) виявилася куди більш цінним джерелом інформації. Вчені діяли за класичним сценарієм зі зрозумілими застереженнями: спочатку був розчинений кальцит - карбонат кальцію, з якого на 97% складається шкаралупа, білкову ж матрицю, що збереглася,  методом хроматографії розділили на фракції, а вже з них виділили ДНК.

До Банса мало хто звертав увагу, що генетичний матеріал насправді може вимиватися з шкаралупи разом з розчинником. Проаналізувавши склад останнього, учені дійсно знайшли в ньому велику кількість ДНК, яка, за їхніми словами, в 125 разів чистіша за видобуту колишнім способом. Відбувається це з тієї причини, що, перебуваючи в шкаралупі, молекула виявляється надійно захищеною від навколишнього середовища і мікроорганізмів.

У результаті такого підходу фахівці змогли виділити ядерну та мітохондріальну ДНК. Серед вивчених таким методом зразків - генетичний код качок з Нової Зеландії, а також ему та сови з Австралії. Найдавніша яєчна шкаралупа належала ему, вимерлого 19 тисяч років тому.

Більше інформації про відкриття можна почерпнути зі ще одного прес-релізу та статті, опублікованій у відкритому доступі журналом Proceedings of the Royal Society B. Нагадаємо, не так давно фахівцям вдалося розшифрувати ДНК мезолітичного тура і орла Хааста.

 

 

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